This site deals with Molecular Genetics and Biochemistry, particularly in relation to genetic mechanisms of biological evolution. The site is searchable using the "Search this blog" box at top left. Blue terms hyperlink to explanatory items so that you may navigate through items providing more detail. The Molecular Genetics Overview and Biochemistry Overview lead into the items. Use the "back" function to return to each departure item.

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Molecular Genetics Overview

Molecular genetics is the study of molecules and mechanisms involved in genetic inheritance. Archival information molecules are long polymers of deoxyribonucleic acid (DNA) comprising bases in specific sequence. The bases adenine (A), thymine (T), cytosine (C), and guanine (G), function as codon triplets – sequences of three bases that code for specific amino acids or for translation initiation (start codons) or termination (stop codons). Uracil is substituted for thymine in RNA.

The segments of DNA that contain protein-coding instructions are called genes, and these gene sequences comprise a portion of the total genome of a cell. The genome includes both the genes (coding-sequences, domains) and the non-coding sequences – both exons, which include open reading frames, and introns.

Because the 64 possible combinations of G-A-T-C code for only the 20 amino acids commonly found in proteins, the code is 'degenerate' (redundant) with more than one triplet combination coding for each amino acid. (This code reduncancy provides hereditary stability by reducing mutation errors.) The double helix of DNA comprises paired nucleotide strands in which the nucleobases are hydrogen bonded to complementary bases in the adjacent chain. Adenine pairs with thymine or uracil (A-TU), and cytosine pairs with guanine (CG).

During cellular reproduction, strands of archival DNA are copied or replicated. Transcription is the first step in gene expression – DNA instructions are converted into mRNA codons, rRNAs, miRNAs, and tRNAs. Coding instructions of nucleotide sequences in archival DNA, which have been transcribed and processed into mRNAs are then translated into polypeptides and proteins at cytoplasmic ribosomes. Translation is the ultimate step in gene expression, in which archival genetic instructions are converted into specified sequences of amino acids in peptides, polypeptides, and proteins.

In prokaryotic cells – without a nuclear membranetranslation into polypeptides and proteins may begin prior to termination of transcription. The molecular genetics of eukaryotic cells is more complicated than that of prokaryotes. Various molecules of ribonucleic acid (RNA) participate in the transcription of the DNA code into processed mRNA in a series of RNA processing stages including capping, polyadenylation, and pre-mRNA splicing.

Following pre-mRNA processing, RNAs undergo extranuclear transfer. Mature RNAs may undergo post-transcriptional modulation (via miRNAs) before translation of the archival DNA instructions into specific sequences of amino acids in the polypeptides and proteins that participate in cellular function and structure. Transfer RNAs (tRNA) deliver specific amino acids to the cytoplasmic ribosomes along the rough endoplasmic reticulum. Ribosomal RNAs participate in assembly of polypeptides and proteins at ribosomes. Here RNAs serve as ribozymes – non-proteinaceous enzymes.

A number of processes are involved in control of cellular function through the maintenance of accuracy of genetic inheritance – damage to DNA is repaired, and faulty RNA is destroyed by nonsense-mediated decay or nonstop decay.

DNA damage may result from replication errors, incorporation of mismatched nucleotides (substitution errors – transitions and transversions), damage by oxygen radicals, hydroxyl radicals, ionizing or ultraviolet radiation, toxins, alkylating agents, and chemotherapy agents. A number of vital mechanisms repair DNA damage to bases (including C to T, C to U, and T U mismatch) and to strands, including double strand breaks. All organisms, prokaryotic and eukaryotic, utilize at least three enzymatic excision-repair mechanisms for damaged bases: base excision repair, mismatch repair, and nucleotide excision repair.

Given the importance of mRNA as an information-carrying molecule, faulty pre-mRNAs and mRNAs must be eliminated – they are destroyed by nonsense-mediated decay or nonstop decay:
1. A pre-mRNA made from a mutant gene usually has an exon junction complex (EJC) in the wrong position. This error activates nonsense-mediated decay (NMD) and destroys the pre-mRNA before it can be used to make flawed proteins. There are at least two kinds of NMD: one requires the protein UPF2 and the other does not.
2. Nonstop decay is mRNA turnover mechanism that has none of the properties of normal mRNA turnover or of NMD. A multi-enzyme complex called the exosome is important for nonstop decay. The exosome is the site for binding of a specific adapter protein called Ski7p. Nonstop decay shares none of the enzymes required for nonsense-mediated decay.

Just as cells repair DNA, they must also maintain the proteome by managing damaged proteins. Heat stress denaturates proteins, causing weakening of polar bonds and exposure of hydrophobic groups. The cellular stress response (heat-shock response) protects organisms from damage resulting from environmental stressors such as heat, UV light, trace metals, and xenobiotics. Stress genes are activated to rapidly synthesize stress proteins, which are highly conserved in biological evolution and play similar roles in organisms from bacteria to humans. Normally, several constitutive stress proteins are present at low levels to function as molecular chaperones, so as to facilitate folding, assembly, and distribution of newly synthesized proteins. For the environmentally stressed cell, stress proteins protect and repair vulnerable protein targets, and play a role in the lysosomal and ubiquitin protein degradation pathways (for removal of unsalvageable proteins). Thus, the cellular stress response performs orchestrated induction of key proteins necessary for cellular protein repair and degradation systems.

Topics: item links : A : adhesion molecules : apoptosis : C : cell biology : chromosomes : D : damage/repair DNA : decay : E : energy : enzymes, specific enzymes : evo-devo : evolution : G : genome : M : mechanisms of evolution : metabolism : molecular genetics : mutation : P : photosynthesis : processing RNA : proteins, specific proteins : R : regulation · cellular differentiation . gene regulation . metabolic regulation : replication : repair/damage DNA : research : RNA : S : serial endosymbiosis theory : signaling : splicing : T : tables : transcription : translation :



actin binding protein

Actin binding proteins (ABP) function in:
● sequestration of actin monomers
● severing actin microfilaments
● capping of actin microfilaments
● cross-linking of microfilaments
● cell motility

Џ Harvard video of actin-myosin action : beautiful Flash 8 animation - Inner Life of the Cell, which shows gelsolin severing and capping an actin filament ; and Interpretation: Inner Life of the Cell Џ


In molecular genetics, an activator is a DNA-binding protein that regulates one or more genes by increasing the rate of transcription. Coactivators act in concert with other transcription factors. CREB binding protein (CBP) is a coactivator-transcription factor that binds with the cAMP response element (CRE).

Activators combine with a gene sequence to up-regulate gene expression. A catabolite gene activator protein (im), also called catabolite gene activator, is a cAMP dependent RNA polymerase that up-regulates gene expression by binding the polymerase at or near the DNA to be transcribed (families). In developmental biology or embryology, activators are substances that stimulate the development of a particular embryonic structure (inductor and organizer).

In biochemistry, an activator is a substance that combines with an enzyme to increase its catalytic activity.

In microbiology, autoinducers are signaling molecules in bacterial quorum sensing that regulate mRNA production for specific genes in response to population density. Autoinducer molecules, which include certain peptides and compounds known as homoserine lactones, can interact with specific repressor or enhancer sequences in DNA. The presence or absence of the autoinducer controls the production of mRNA, and therefore regulates gene expression into protein.


adenylate cyclases

Adenylyl (adenylate) cyclases are transmembrane enzymes that cross the membrane twelve times, and which convert ATP to the second-messenger cAMP (3',5' cyclic AMP) and pyrophosphate (Pi). Likewise, guanylate cyclases convert GTP to the second messenger, cGMP.

Adenylyl cyclases are coincidence detectors, meaning that they are only activated by several different signals occurring together – they are modulated by G-proteins, forskolin, Ca2+/calmodulin, and other class-specific substrates.

Glossary : A . B . C . D . E . F . G . H . I . J . K . L . M . N . O . P . Q . R . S . T . U . V . W . X . Y . Z .

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